Rapid and facile detection of HBV with CRISPR/Cas13a-Reference-Cited by-同舟云学术

Rapid and facile detection of HBV with CRISPR/Cas13a

Published:2022 Issue:41 Volume:46 Page:19997-20004
ISSN:1144-0546
Container-title:New Journal of Chemistry
language:en
Short-container-title:New J. Chem.

Author:

Ding Ronghua¹²,Shen Yue¹,Yuan Mingzhu¹,Zheng Xue¹,Chen Shuaiyin¹^ORCID,Duan Guangcai¹³

Affiliation:

1. Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou 450000, Henan, China

2. Jining No. 1 People′s Hospital, Jining 272000, Shandong, China

3. Key Laboratory of Molecular Medicine in Henan Province, Zhengzhou University, Zhengzhou 450000, Henan, China

Abstract

A rapid point-of-care assay based on CRISPR-Cas13a for HBV was built with a LOD of 1 copy per μL was achieved in 50 min. The lateral flow test strip method can achieve naked-eye results, and the fluorescence readout can achieve real-time detection.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,General Chemistry,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/NJ/D2NJ02674K

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