Artificial intelligence-based classification of peripheral blood nucleated cells using label-free imaging flow cytometry-Reference-Cited by-同舟云学术

Artificial intelligence-based classification of peripheral blood nucleated cells using label-free imaging flow cytometry

Published:2022 Issue:18 Volume:22 Page:3464-3474
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Hirotsu Amane¹,Kikuchi Hirotoshi¹^ORCID,Yamada Hidenao²^ORCID,Ozaki Yusuke¹,Haneda Ryoma¹,Kawata Sanshiro¹,Murakami Tomohiro¹,Matsumoto Tomohiro¹,Hiramatsu Yoshihiro³^ORCID,Kamiya Kinji¹,Yamashita Daisuke²,Fujimori Yuki²,Ueda Yukio²,Okazaki Shigetoshi⁴,Kitagawa Masatoshi⁵⁶,Konno Hiroyuki⁷,Takeuchi Hiroya¹

Affiliation:

1. Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan

2. Central Research Laboratory, Hamamatsu Photonics K.K, Hamamatsu, Shizuoka, Japan

3. Department Perioperative Functioning Care and Support, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan

4. HAMAMATSU BioPhotonics Innovation Chair, Institute for Medical Photonics Research, Preeminent Medical Photonics Education and Research Centre, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan

5. Department of Molecular Biology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan

6. Laboratory Animal Facilities and Services, Preeminent Medical Photonics Education and Research Centre, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan

7. Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan

Abstract

We developed a method for label-free image identification and classification of peripheral blood nucleated cells flowing in a microfluidic channel, based on the subcellular structures of quantitative phase microscopy images.

Funder

Japan Agency for Medical Research and Development

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D2LC00166G

Reference38 articles.

1. Role of fibrocytes and endothelial progenitor cells among low-differentiated CD34+ cells in the progression of lung sarcoidosis

2. Circulating Endothelial Cells