Facile quantitative comparison of specific activities of fusion-tagged enzyme/mutants in cell lysates via prediction of their maximum adsorption by anti-tag antibody immobilized in microplate wells-Reference-Cited by-同舟云学术

Facile quantitative comparison of specific activities of fusion-tagged enzyme/mutants in cell lysates via prediction of their maximum adsorption by anti-tag antibody immobilized in microplate wells

Published:2014 Issue:56 Volume:4 Page:29925-29932
ISSN:2046-2069
Container-title:RSC Adv.
language:en
Short-container-title:RSC Adv.

Author:

Li Y. L.¹²³⁴⁵,Yang X. L.¹²³⁴⁵,He C. X.¹²³⁴⁵,Hu X. L.¹²³⁴⁵,Pu J.¹²³⁴⁵,Liu L.¹²³⁴⁵,Long G. B.¹²³⁴⁵,Liao F.¹²³⁴⁵

Affiliation:

1. Unit for Analytical Probes and Protein Biotechnology

2. Key Laboratory of Clinical Laboratory Diagnostics of the Education Ministry

3. College of Laboratory Medicine

4. Chongqing Medical University

5. Chongqing 400016, China

Abstract

Maximum activities of 6His-tagged enzyme/mutants from lysates adsorbed on immobilized anti-tag antibody were predicted as specific activities for comparison.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/RA/C4RA03189J

Reference18 articles.

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