Interactions of the primers and Mg2+with graphene quantum dots enhance PCR performance-Reference-Cited by-同舟云学术

Interactions of the primers and Mg2+with graphene quantum dots enhance PCR performance

Published:2015 Issue:91 Volume:5 Page:74515-74522
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Zhu Meidong¹²³⁴⁵,Luo Chao¹²³⁴⁵,Zhang Fangwei⁶⁷⁵⁸,Liu Fei¹²³⁴⁵,Zhang Jingyan¹²³⁴⁵,Guo Shouwu⁶⁷⁵⁸

Affiliation:

1. State Key Laboratory of Bioreactor Engineering

2. Shanghai Key Laboratory of New Drug Design

3. School of Pharmacy

4. East China University of Science and Technology

5. Shanghai

6. School of Electronic Information and Electrical Engineering

7. Shanghai Jiao Tong University

8. P. R. China

Abstract

GQDs enhance PCR performance through stacking the primers selectively, tuning the activity of polymeraseviachelating Mg²⁺, and accelerating the PCR reaction by adsorbing PCR reaction components together to increase their proximity.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/RA/C5RA12729G

Reference37 articles.

1. Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

2. Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

3. Formamide can dramatically improve the specificity of PCR

4. Dynamic passivation with BSA overcomes LTCC mediated inhibition of PCR

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