Chemical synthesis of stimuli-responsive guide RNA for conditional control of CRISPR-Cas9 gene editing-Reference-Cited by-同舟云学术

Chemical synthesis of stimuli-responsive guide RNA for conditional control of CRISPR-Cas9 gene editing

Published:2021 Issue:29 Volume:12 Page:9934-9945
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Gu Chunmei¹²³⁴⁵,Xiao Lu¹²³⁴⁵,Shang Jiachen¹²³⁴⁵,Xu Xiao¹²³⁴⁵,He Luo¹²³⁴⁵,Xiang Yu¹²³⁴⁵^ORCID

Affiliation:

1. Department of Chemistry

2. Beijing Key Laboratory for Microanalytical Methods and Instrumentation

3. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education)

4. Tsinghua University

5. Beijing 100084

Abstract

Conditional control of CRISPR-Cas9 activity by reactive oxygen species and visible light is achieved using stimuli-responsive guide RNA synthesized by a general method based on RNA 2′-O-methylribonucleotide phosphorothioate.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D1SC01194D

Reference90 articles.

1. Applications of CRISPR technologies in research and beyond

2. Multiplex Genome Engineering Using CRISPR/Cas Systems

3. RNA-Guided Human Genome Engineering via Cas9

4. RNA-programmed genome editing in human cells

5. Delivery technologies for genome editing

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