HAMA: a multiplexed LC-MS/MS assay for specificity profiling of adenylate-forming enzymes
Author:
Affiliation:
1. Independent Junior Research Group Biosynthetic Design of Natural Products
2. Leibniz Institute for Natural Product Research and Infection Biology e.V.
3. Hans Knöll Institute (HKI Jena)
4. 07745 Jena
5. Germany
Abstract
Adenylation enzymes are engineering targets in ribosomal and nonribosomal peptide synthesis. Through multiplexed LC-MS/MS measurement of hydroxamates, the HAMA assay records specificity profiles of these enzymes in a snap.
Funder
Daimler und Benz Stiftung
Publisher
Royal Society of Chemistry (RSC)
Subject
General Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2019/SC/C9SC04222A
Reference41 articles.
1. Structural basis of the nonribosomal codes for nonproteinogenic amino acid selective adenylation enzymes in the biosynthesis of natural products
2. Adenylate-forming enzymes
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4. Adding New Chemistries to the Genetic Code
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