Quantitative analysis of biochemical processes in living cells at a single-molecule level: a case of olaparib–PARP1 (DNA repair protein) interactions-Reference-Cited by-同舟云学术

Quantitative analysis of biochemical processes in living cells at a single-molecule level: a case of olaparib–PARP1 (DNA repair protein) interactions

Published:2021 Issue:23 Volume:146 Page:7131-7143
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Karpińska Aneta¹^ORCID,Pilz Marta¹^ORCID,Buczkowska Joanna¹^ORCID,Żuk Paweł J.¹²^ORCID,Kucharska Karolina¹^ORCID,Magiera Gaweł³^ORCID,Kwapiszewska Karina¹^ORCID,Hołyst Robert¹^ORCID

Affiliation:

1. Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland

2. Department of Physics, Lancaster University, Lancaster LA1 4YB, UK

3. Department of Medicine, Poznan University of Medical Sciences, 60-356, Poznan, Poland

Abstract

Fluorescence correlation spectroscopy was applied to quantify drug-target interactions directly in living human cells.

Funder

Narodowe Centrum Badań i Rozwoju

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN01769A

Reference47 articles.

1. Recent advances in FRET for the study of protein interactions and dynamics

2. Analytical methods for kinetic studies of biological interactions: A review

3. Determination of oligomerization state of Drp1 protein in living cells at nanomolar concentrations

4. Quantifying Nanoscale Viscosity and Structures of Living Cells Nucleus from Mobility Measurements

5. Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines

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