A rapid and concise setup for the fast screening of FRET pairs using bioorthogonalized fluorescent dyes-Reference-Cited by-同舟云学术

A rapid and concise setup for the fast screening of FRET pairs using bioorthogonalized fluorescent dyes

Published:2018 Issue:16 Volume:16 Page:2997-3005
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Petrovics Réka¹²³⁴⁵,Söveges Bianka¹²³⁴⁵,Egyed Alexandra¹²³⁴⁵,Knorr Gergely¹²³⁴⁵,Kormos Attila¹²³⁴⁵^ORCID,Imre Tímea¹⁶⁷⁴⁵,Török György¹⁸⁹⁴⁵^ORCID,Zeke András¹⁸¹⁰⁴⁵,Kocsmár Éva¹¹¹²¹³⁵,Lotz Gábor¹¹¹²¹³⁵,Kele Péter¹²³⁴⁵^ORCID,Németh Krisztina¹²³⁴⁵^ORCID

Affiliation:

1. Research Centre for Natural Sciences of Hungarian Academy of Sciences

2. Institute of Organic Chemistry

3. “Lendület” Chemical Biology Research Group

4. H-1117 Budapest

5. Hungary

6. Instrumentation Center

7. MS Metabolomics Research Group

8. Institute of Enzymology

9. Molecular Cell Biology Research Group

10. Protein Research Group

11. 2nd Department of Pathology

12. Semmelweis University

13. Budapest H-1091 Üllői str. 93

Abstract

Fluorescent dyes for bioorthogonal labeling were paired (Cy1A, Cy3T, Cy5T, CBRD1A) based on spectral characteristics, and high efficiency FRET application is reported.

Funder

Magyar Tudományos Akadémia

Országos Tudományos Kutatási Alapprogramok

Emberi Eroforrások Minisztériuma

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/OB/C8OB00213D

Reference39 articles.

1. Analysis of protein interactions using fluorescence technologies