Directed evolution of C-methyltransferase PsmD for enantioselective pyrroloindole derivative production-Reference-Cited by-同舟云学术

Directed evolution of C-methyltransferase PsmD for enantioselective pyrroloindole derivative production

Published:2024 Issue: Volume: Page:
ISSN:2044-4753
Container-title:Catalysis Science & Technology
language:en
Short-container-title:Catal. Sci. Technol.

Author:

Amariei Diana A.¹,Tenhaef Julia²^ORCID,Classen Thomas²^ORCID,David Benoit³,Rosch Tobias M.²^ORCID,Gohlke Holger³⁴^ORCID,Noack Stephan²^ORCID,Pietruszka Jörg¹²^ORCID

Affiliation:

1. Institute of Bioorganic Chemistry & Bioeconomy Science Center (BioSC), Heinrich Heine University Düsseldorf in Forschungszentrum Jülich, Jülich, Germany

2. Institute of Bio- and Geosciences (IBG-1: Biotechnology), Forschungszentrum Jülich, Jülich, Germany

3. Institute of Bio- and Geosciences (IBG-4: Bioinformatics), Forschungszentrum Jülich, Jülich, Germany

4. Institute for Pharmaceutical and Medicinal Chemistry & Bioeconomy Science Center (BioSC), Heinrich Heine University Düsseldorf, Düsseldorf, Germany

Abstract

The engineering of stereoselective C-methyltransferase PsmD through saturation mutagenesis led to improved activity for larger substrates. An automated process was designed and successfully applied for the mutant library production and screening.

Funder

Heinrich-Heine-Universität Düsseldorf

Forschungszentrum Jülich

Bundesministerium für Bildung und Forschung

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CY/D4CY00657G

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