NMR Study of the Structure and Dynamics of Chaperone–Client Complexes

Abstract

Recent technical developments in solution NMR have increased the understanding of molecular chaperones. Increased structural information on chaperone–client protein complexes at atomic resolution has unveiled the mechanisms underlying the recognition of client proteins by molecular chaperones. Furthermore, understanding of the dynamics and kinetics of chaperone–client complexes has highlighted the effect of chaperones on the folding properties of client proteins. This chapter summarizes recent advancements in NMR studies of molecular chaperones and chaperone–client protein complexes.