Chronobiological Study Designs

Abstract

The chapter describes experimental designs for various chronobiological studies aimed at basic research and clinical trials, with an emphasis on circadian rhythms. In the first part, various methods of data collection, particularly longitudinal and transverse sampling and their relative merits, are discussed. Thereafter, specific methods and their constraints for monitoring marker rhythms are presented. Variables that are most effective in characterizing the endogenous pacemaker and those of clinical relevance are discussed. Besides melatonin and core body temperature rhythms, which are widely accepted as the gold standard for representing the circadian clock, rhythms of cortisol concentration, physical activity, sleep parameters and chronotypes are considered. The relevance of stable rhythms with appropriate internal and external phase relationships for health and wellbeing, as well as adverse effects of certain rhythm alterations are discussed. The last part describes two experimental designs that allow separating endogenous and exogenous components of biological rhythms, the constant routine and the forced desynchronization protocols.