A fluorescent probe for specifically measuring the overall thioredoxin and glutaredoxin reducing activity in bacterial cells-Reference-Cited by-同舟云学术

A fluorescent probe for specifically measuring the overall thioredoxin and glutaredoxin reducing activity in bacterial cells

Published:2022 Issue:5 Volume:147 Page:834-840
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zuo Xin¹,Zhao Ying¹,Zhao Jintao²^ORCID,Ouyang Yanfang¹,Qian Wenjun¹,Hou Yinmei¹,Yu Chong¹,Ren Xiaoyuan³,Zou Lili⁴^ORCID,Fang Jianguo²,Lu Jun¹^ORCID

Affiliation:

1. Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education (Southwest University), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

2. State Key Laboratory of Applied Organic Chemistry & College of Chemistry and Chemical Engineering, Lanzhou University, China

3. Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden

4. The Institute of Infection and Inflammation, Medical College, China Three Gorges University, 443000 Yichang, China

Abstract

Both bacterial thioredoxin and glutaredoxin systems can reduce TRFS-green selectively, which confers TRFS-green to be a remarkable probe to detect the dominant disulfide reductase activity with a slow reaction rate in bacteria, e. g. E. coli Grx2&3.

Funder

Natural Science Foundation of Chongqing

Southwest University

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AN/D1AN01644J

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1. Oxidative Stress

2. Redox Biology on the rise