Spatially resolved quantification of oxygen consumption rate in <i>ex vivo</i> lymph node slices-Reference-Cited by-同舟云学术

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

Published:2024 Issue:9 Volume:149 Page:2609-2620
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Anbaei Parastoo¹,Stevens Marissa G.²³,Ball Alexander G.⁴³,Bullock Timothy N. J.²³,Pompano Rebecca R.¹⁵³^ORCID

Affiliation:

1. Department of Chemistry, University of Virginia College of Arts and, Sciences, Charlottesville, Virginia 22904, USA

2. Department of Pathology, University of Virginia, Charlottesville, Virginia 22903, USA

3. Carter Immunology Center and UVA Cancer Center, University of Virginia, Charlottesville, Virginia 22903, USA

4. Department of Microbiology Cancer Biology and Immunology, University of Virginia, Charlottesville, Virginia 22903, USA

5. Department of Biomedical Engineering, University of Virginia School of Engineering and Applied Sciences, Charlottesville, Virginia 22904, USA

Abstract

A spatially resolved optical method was developed to measure oxygen consumption rate (OCR) in ex vivo tissue slices. This method revealed regional OCR variations and is a valuable tool for exploring tissue hypoxia dynamics and immune cell activity.

Funder

National Institute of Allergy and Infectious Diseases

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D4AN00028E

Reference51 articles.

1. HIF-1 stabilization in T cells hampers the control of Mycobacterium tuberculosis infection

2. HIF-1α pathway: role, regulation and intervention for cancer therapy

3. Targeting HIF-1 alpha transcriptional activity drives cytotoxic immune effector cells into melanoma and improves combination immunotherapy

4. Germinal centre hypoxia and regulation of antibody qualities by a hypoxia response system

5. Germinal Center Hypoxia Potentiates Immunoglobulin Class Switch Recombination