Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing-Reference-Cited by-同舟云学术

Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing

Published:2014 Issue:57 Volume:50 Page:7597-7600
ISSN:1359-7345
Container-title:Chem. Commun.
language:en
Short-container-title:Chem. Commun.

Author:

Chen Xiong¹²³,El-Sagheer Afaf H.⁴⁵⁶⁷⁸,Brown Tom⁹¹⁰¹¹¹²

Affiliation:

1. School of Chemistry

2. University of Southampton

3. Southampton SO17 1BJ, UK

4. Chemistry Branch

5. Dept. of Science and Mathematics

6. Faculty of Petroleum and Mining Engineering

7. Suez University

8. Suez, Egypt

9. Department of Chemistry

10. University of Oxford

11. Chemistry Research Laboratory

12. Oxford, UK

Abstract

Reverse-transcriptase reads through the bulky artificial triazole linkage with omission of one nucleotide with implications for RNA isolation and analysis.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2014/CC/C4CC03027C

Reference22 articles.

1. Origins and Mechanisms of miRNAs and siRNAs

2. The Next-Generation Sequencing Revolution and Its Impact on Genomics

3. Low-bias, strand-specific transcriptome Illumina sequencing by on-flowcell reverse transcription (FRT-seq)

4. Limitations and possibilities of small RNA digital gene expression profiling

5. RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries

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