Linear-hairpin variable primer RT-qPCR for MicroRNA-Reference-Cited by-同舟云学术

Linear-hairpin variable primer RT-qPCR for MicroRNA

Published:2019 Issue:7 Volume:10 Page:2034-2043
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Lan Lin¹²³⁴,Guo Qiuping¹²³⁴⁵,Nie Hemin¹²³⁴^ORCID,Zhou Chang⁶⁷³⁴,Cai Qingyun⁸²³⁴⁵^ORCID,Huang Jin⁸²³⁴⁵^ORCID,Meng Xiangxian¹²³⁴

Affiliation:

1. College of Biology

2. Hunan University

3. Changsha

4. P. R. China

5. State Key Laboratory of Chemo/Biosensing and Chemometrics

6. School of Life Sciences

7. Hunan Normal University

8. College of Chemistry and Chemical Engineering

Abstract

Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/SC/C8SC04621B

Reference34 articles.

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5. MicroRNAs—Future Drug Targets?

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