SNAP-tagging live cells <i>via</i> chelation-assisted copper-catalyzed azide–alkyne cycloaddition-Reference-Cited by-同舟云学术

登录注册会员服务联系我们

SNAP-tagging live cells via chelation-assisted copper-catalyzed azide–alkyne cycloaddition

Published:2023 Issue:36 Volume:21 Page:7419-7436
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Stone Daniel J.¹,Macias-Contreras Miguel¹^ORCID,Crist Shaun M.¹,Bucag Christelle F. T.¹,Seo Gwimoon²,Zhu Lei¹^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390, USA

2. Institute of Molecular Biophysics, Florida State University, 91 Chieftan Way, Tallahassee, FL 32306-4380, USA

Abstract

The sequential biochemical (SNAP-tag) and chemical (chelation-assisted copper-catalyzed azide–alkyne cycloaddition) reactions are applied in membrane protein labeling on live cells. The second, chemical step is rapid (within 1 minute) without any ill-effect to the labeled cells.

Funder

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/OB/D3OB01003A

Reference79 articles.

1. A general method for the covalent labeling of fusion proteins with small molecules in vivo

2. Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging

3. Measuring In Vivo Protein Half-Life

4. Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370

www.globalauthorid.com

TOP