SNAP-tagging live cells <i>via</i> chelation-assisted copper-catalyzed azide–alkyne cycloaddition-Reference-Cited by-同舟云学术

SNAP-tagging live cells via chelation-assisted copper-catalyzed azide–alkyne cycloaddition

Published:2023 Issue:36 Volume:21 Page:7419-7436
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Stone Daniel J.¹,Macias-Contreras Miguel¹^ORCID,Crist Shaun M.¹,Bucag Christelle F. T.¹,Seo Gwimoon²,Zhu Lei¹^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390, USA

2. Institute of Molecular Biophysics, Florida State University, 91 Chieftan Way, Tallahassee, FL 32306-4380, USA

Abstract

The sequential biochemical (SNAP-tag) and chemical (chelation-assisted copper-catalyzed azide–alkyne cycloaddition) reactions are applied in membrane protein labeling on live cells. The second, chemical step is rapid (within 1 minute) without any ill-effect to the labeled cells.

Funder

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/OB/D3OB01003A

Reference79 articles.

1. A general method for the covalent labeling of fusion proteins with small molecules in vivo

2. Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging

3. Measuring In Vivo Protein Half-Life

4. Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro

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