Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR-Reference-Cited by-同舟云学术

Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR

Published:2023 Issue:1 Volume:59 Page:102-105
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Eladl Omar¹²³^ORCID,Yamaoki Yudai¹²^ORCID,Kondo Keiko¹^ORCID,Nagata Takashi¹²^ORCID,Katahira Masato¹²^ORCID

Affiliation:

1. Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

2. Graduate School of Energy Science, Kyoto University, Yoshida-hommachi, Sakyo-ku, Kyoto 606-8501, Japan

3. Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt

Abstract

A labeled RNA aptamer prepared with E. coli was introduced into human cells. Aptamer interaction was investigated using 2D in-cell NMR.

Funder

Japan Society for the Promotion of Science

Japan Agency for Medical Research and Development

Osaka University

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2023/CC/D2CC05576G

Reference29 articles.

1. Structural basis of RNA folding and recognition in an AMP–RNA aptamer complex

2. Structural Basis of the Highly Efficient Trapping of the HIV Tat Protein by an RNA Aptamer

3. Selection of RNA Aptamers that Bind Specifically to the NS3 Protease of Hepatitis C Virus

4. Identification of Liver Cancer-Specific Aptamers Using Whole Live Cells

5. Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

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