CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H-Reference-Cited by-同舟云学术

CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H

Published:2022 Issue: Volume: Page:
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Kim Hansol¹,Lee Seoyoung¹,Lee Jinhwan¹,Park Hyun Gyu¹^ORCID

Affiliation:

1. Department of Chemical and Biomolecular Engineering (BK 21+ program), Korea Advanced Institute of Science and Technology (KAIST), Daehak-ro 291, Yuseong-gu, Daejeon 34141, Republic of Korea

Abstract

We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL−1.

Funder

National Research Foundation of Korea

Korean National Police Agency

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CC/D1CC06026K

Reference36 articles.

1. Properties of Cloned and Expressed Human RNase H1

2. Ribonuclease H: the enzymes in eukaryotes

3. Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli

4. Catalytic center of an archaeal type 2 ribonuclease H as revealed by X-ray crystallographic and mutational analyses

5. Ribonuclease H, an unexploited target for antiviral intervention against HIV and hepatitis B virus

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension reaction;Biosensors and Bioelectronics;2024-06

2. CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics;Environmental Technology & Innovation;2024-05

3. An ultrasensitive label-free RNase H assay based on in vitro transcription of fluorogenic light-up aptamer;Nanoscale Advances;2024

4. CRISPR/Cas12a Collateral Cleavage Activity for Sensitive 3′–5′ Exonuclease Assay;Biosensors;2023-10-30

5. Reverse Transcriptase Enzyme-assisted recombinant isothermal amplification with CRISPR/Cpf1 for RNA virus assay;Process Biochemistry;2023-07