An automated system for interrogating the evolution of microbial endosymbiosis

Author:

Huang Can1ORCID,Guo Fengguang2,Wang Han3ORCID,Olivares Jasmine2,Dalton, III James2,Belyanina Olga2,Wattam Alice R.4,Cucinell Clark A.4,Dickerman Allan W.4,Qin Qing-Ming2,Han Arum156ORCID,de Figueiredo Paul27

Affiliation:

1. Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA

2. Department of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, Bryan, TX 77843, USA

3. Department of Biomedical Engineering, School of Medicine, Tsinghua University, 100084, Beijing, China

4. Biocomplexity Institute and Initiative, University of Virginia, Charlottesville, VA 22904, USA

5. Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA

6. Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX, 77843, USA

7. Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USA

Abstract

Using an automated microfluidic SEER platform, we rapidly evolved laboratory naïve E. coli strain DH5α to possess enhanced capability to survive within host cells, and identified the critical role of gene CpxR in such microbial endosymbiosis.

Funder

Army Research Laboratory

Bill and Melinda Gates Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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