Bioluminescence assay for rapid detection of live Staphylococcus aureus based on the enrichment of egg yolk antibody modified magnetic metal organic framework immunobeads

Author:

Zeng Mengli12,Zhou Renjie1,He Tingting1,Hu Fanling3,Liu Weiyue4,Gan Ning1ORCID,Yu Shaoning1

Affiliation:

1. Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang province, 315211, China

2. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China

3. The Barstow School Ningbo Campus, Ningbo, 315211, China

4. School of Information Science and Engineering, Ningbo University, Ningbo 315211, China

Abstract

The main significance and novelty can be summarized: (1) mMOF-IgY efficiently enrich S.A and amplify bioluminescence signal. (2) Lysed S.A emit ATP and produce bioluminescence. (3) The assay can detect S.A in foods with LOD of 3 CFU mL−1 in 30 min.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Zhejiang Province

K. C. Wong Magna Fund in Ningbo University

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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