Simple and convenient G-quadruplex-based fluorescence assay of micrococcal nuclease activity
Author:
Affiliation:
1. Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing)
2. Ministry of Agriculture
3. Citrus Research Institute
4. Southwest University
5. Chongqing
Abstract
An ultrasensitive and straightforward fluorescence sensing platform for MNase activity has been developed based on NMM/G-quadruplexes.
Funder
National Natural Science Foundation of China
Publisher
Royal Society of Chemistry (RSC)
Subject
General Chemical Engineering,General Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2015/RA/C5RA20773H
Reference40 articles.
1. The use of micrococcal nuclease as a probe for drug-binding sites on DNA
2. Micrococcal nuclease cleavage of nucleotide linked to glutamine synthetase yields phosphotyrosine at the ligation site.
3. Analysis of DNA double- and single-strand breaks by two dimensional electrophorresis: action of micrococcal nuclease on chromatin and DNA, and degradation in vivo of lens fiber chromatin
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