Hyperconjugation promoted by hydrogen bonding between His98/His241 and a carboxyl group contributes to tyrosine decarboxylase catalysis

Author:

Ni Jie12345,Xu Guochao12345,Dai Wei12345,Zhao Yi-Lei678910ORCID,Ni Ye12345ORCID

Affiliation:

1. Key Laboratory of Industrial Biotechnology

2. Ministry of Education

3. School of Biotechnology

4. Jiangnan University

5. Wuxi 214122

6. State Key Laboratory of Microbial Metabolism

7. Joint International Research Laboratory of Metabolic and Developmental Sciences

8. MOE-LSB and MOE-LSC

9. School of Life Sciences and Biotechnology

10. Shanghai Jiao Tong University

Abstract

This article first demonstrates the utility of the σ → π* hyperconjugation by His98 and His241 in pyridoxal 5′-phosphate-dependent tyrosine decarboxylase.

Funder

National Basic Research Program of China

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Catalysis

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