Development of a 1,2-difluorofucoside activity-based probe for profiling GH29 fucosidases-Reference-Cited by-同舟云学术

Development of a 1,2-difluorofucoside activity-based probe for profiling GH29 fucosidases

Published:2021 Issue:13 Volume:19 Page:2968-2977
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Luijkx Yvette M. C. A.¹²³⁴⁵,Jongkees Seino¹²³⁴⁵,Strijbis Karin⁶⁷⁸³⁴,Wennekes Tom¹²³⁴⁵^ORCID

Affiliation:

1. Department Chemical Biology and Drug Discovery

2. Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research

3. Utrecht University

4. Utrecht

5. The Netherlands

6. Department Biomolecular Health Sciences

7. Division Infectious diseases and Immunology

8. Faculty of Veterinary Medicine

Abstract

We developed the activity-based probe YL209 that can covalently functionalize diverse GH29 α-l-fucosidases and detect these at nanogram levels after a click reaction with a biotin reporter.

Funder

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

H2020 European Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/OB/D1OB00054C

Reference51 articles.

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4. Identifying the Catalytic Acid/Base in GH29 α-l-Fucosidase Subfamilies

5. Substrate specificity and other properties of alpha-L-fucosidase from human serum.

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