Key role of cycloalkyne nature in alkyne-dye reagents for enhanced specificity of intracellular imaging by bioorthogonal bioconjugation-Reference-Cited by-同舟云学术

Key role of cycloalkyne nature in alkyne-dye reagents for enhanced specificity of intracellular imaging by bioorthogonal bioconjugation

Published:2024 Issue: Volume: Page:
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Vidyakina Alexandra A.¹^ORCID,Silonov Sergey A.¹²^ORCID,Govdi Anastasia I.¹^ORCID,Ivanov Alexander Yu.³^ORCID,Podolskaya Ekaterina P.⁴,Balova Irina A.¹^ORCID,Bräse Stefan⁵⁶^ORCID,Danilkina Natalia A.¹^ORCID

Affiliation:

1. Institute of Chemistry, Saint Petersburg State University (SPbU), Saint Petersburg, 199034, Russia

2. Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 194064, Russia

3. Center for Magnetic Resonance, Research Park, Saint Petersburg State University (SPbU), Saint Petersburg, 199034, Russia

4. Institute of Analytical Instrumentation RAS, 190103 St. Petersburg, Russia

5. Institute of Organic Chemistry (IOC), Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany

6. Institute of Biological and Chemical Systems – Functional Molecular Systems (IBCS-FMS), Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany

Abstract

Benzothiophene-fused azacyclononyne-dye reagents were created to demonstrate that a less reactive cycloalkyne is preferable to known highly reactive DBCO and BCN in the design of SPAAC intracellular bioimaging reagents.

Funder

Russian Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/OB/D4OB01032A

Reference51 articles.

1. Chemistry in living systems

2. Mechanisms and Substituent Effects of Metal-Free Bioorthogonal Reactions

3. Developing bioorthogonal probes to span a spectrum of reactivities

4. Bioorthogonal chemistry mediated cell engineering for advanced cell and cell-derived vesicle therapies: Principles, progresses, and remaining challenges

5. Bioorthogonal Chemistry and Its Applications