Sophisticated and precise: design and implementation of a real-time optical detection system for ultra-fast PCR-Reference-Cited by-同舟云学术

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Sophisticated and precise: design and implementation of a real-time optical detection system for ultra-fast PCR

Published:2023 Issue:29 Volume:13 Page:19770-19781
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Xi Bangchao¹²³,Huang Shaolei¹²³,An Yiquan¹²³,Gong Xianglian¹²³,Yang Jiayu¹²³,Zeng Juntian¹²³,Ge Shengxiang¹²³,Zhang Dongxu¹²³^ORCID

Affiliation:

1. State Key Laboratory of Molecular Vaccinology and Molecular Diagnostic (Xiamen University), Xiamen 361102, Fujian, China

2. National Institute of Diagnostics and Vaccine Development in Infection Diseases (Xiamen University), Xiamen 361102, Fujian, China

3. School of Public Health, Xiamen University, Xiamen 361102, Fujian, China

Abstract

Proposes a real-time fluorescence detection system for ultra-fast PCR that lifts limitations and significantly improves overall performance.

Funder

National Natural Science Foundation of China

Science and Technology Projects of Fujian Province

Xiamen University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/RA/D3RA03363E

Reference33 articles.

1. Ultra-rapid real-time microfluidic RT-PCR instrument for nucleic acid analysis

2. Under-three minute PCR: Probing the limits of fast amplification

3. Ultrafast Rotary PCR system for multiple influenza viral RNA detection

4. Ultrafast photonic PCR

5. Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

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1. Physical Simulation-Based Calibration for Quantitative Real-Time PCR;Applied Sciences;2024-06-09

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