A ddPCR platform based on a microfluidic chip with a dual-function flow-focusing structure for sample-to-result DNA quantification analysis-Reference-Cited by-同舟云学术

A ddPCR platform based on a microfluidic chip with a dual-function flow-focusing structure for sample-to-result DNA quantification analysis

Published:2024 Issue: Volume: Page:
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Zhang Xiaoliang¹²^ORCID,Wang Shun²,Wang Jinxian¹²^ORCID,Sun Xiaojie¹²,Xue Jinbing²,Wang Zhenya²,Yang Tianhang²,Weng Liangfei³,Wang Bidou¹²,Luo Gangyin¹²^ORCID

Affiliation:

1. School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, People's Republic of China

2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, People's Republic of China

3. Suzhou Guoke Medical Science & Technology Development Co. Ltd, Suzhou 215163, People's Republic of China

Abstract

A sample-to-result ddPCR platform based on a microfluidic chip with a dual-function flow-focusing structure enabling droplet generation via forward flow and droplet readout via reflux by rotating the rotary valve was developed.

Funder

Chinese Academy of Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2024/LC/D3LC01078C

Reference25 articles.

1. Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

2. EGFR C797S, EGFR T790M and EGFR sensitizing mutations in non-small cell lung cancer revealed by six-color crystal digital PCR

3. A review on continuous-flow microfluidic PCR in droplets: Advances, challenges and future

4. Digital and Absolute Assays for Low Abundance Molecular Biomarkers

5. Digital PCR