A ddPCR platform based on a microfluidic chip with a dual-function flow-focusing structure for sample-to-result DNA quantification analysis

Author:

Zhang Xiaoliang12ORCID,Wang Shun2,Wang Jinxian12ORCID,Sun Xiaojie12,Xue Jinbing2,Wang Zhenya2,Yang Tianhang2,Weng Liangfei3,Wang Bidou12,Luo Gangyin12ORCID

Affiliation:

1. School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, People's Republic of China

2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, People's Republic of China

3. Suzhou Guoke Medical Science & Technology Development Co. Ltd, Suzhou 215163, People's Republic of China

Abstract

A sample-to-result ddPCR platform based on a microfluidic chip with a dual-function flow-focusing structure enabling droplet generation via forward flow and droplet readout via reflux by rotating the rotary valve was developed.

Funder

Chinese Academy of Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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