Development of a PNA–DiSc<sub>2</sub> based portable absorbance platform for the detection of pathogen nucleic acids-Reference-Cited by-同舟云学术

Development of a PNA–DiSc₂ based portable absorbance platform for the detection of pathogen nucleic acids

Published:2022 Issue:23 Volume:147 Page:5306-5313
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Lad Shailesh B.¹,Roy Shomdutta¹,George Jijo Easo¹,Chakraborti Himadri²,Lalsare Saumitra¹,Barik Bikash²,Singh Arushi²,Zade Amrutraj³,Agrawal Sachee⁴,Shastri Jayanthi⁴,Chatterjee Anirvan³,Das Gupta Kantimay²^ORCID,Paul Debjani¹^ORCID,Kondabagil Kiran¹^ORCID

Affiliation:

1. Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India

2. Department of Physics, Indian Institute of Technology Bombay, Powai, Mumbai, India

3. Haystack Analytics Pvt. Ltd. Society For Innovation & Entrepreneurship (SINE), Kanwal Rekhi Building, Indian Institute of Technology Bombay, Powai, Mumbai, India

4. Molecular Diagnostic Reference Laboratory, Kasturba Hospital for Infectious Diseases, Mumbai, India

Abstract

A PortAbs based pathogen nucleic acid detection system using peptide nucleic acid (PNA) and a cyanine dye, DiSc2(5). The shift is measured of the absorbance induced by binding of a PNA probe to a complementary DNA strand using a portable two-color absorption system.

Funder

Indian Institute of Technology Bombay

Department of Biotechnology, Ministry of Science and Technology, India

Department of Science and Technology, Ministry of Science and Technology, India

Council of Scientific and Industrial Research, India

University Grants Commission

Science and Engineering Research Board

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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