A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(iii)-based i-motif probe-Reference-Cited by-同舟云学术

A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(iii)-based i-motif probe

Published:2015 Issue:49 Volume:51 Page:9953-9956
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Lu Lihua¹²³⁴,Wang Modi¹²³⁴,Liu Li-Juan⁵⁶⁷⁸⁴,Wong Chun-Yuen⁹¹⁰⁴,Leung Chung-Hang⁵⁶⁷⁸⁴,Ma Dik-Lung¹²³⁴¹¹

Affiliation:

1. Department of Chemistry

2. Hong Kong Baptist University

3. Hong Kong

4. China

5. State Key Laboratory of Quality Research in Chinese Medicine

6. Institute of Chinese Medical Sciences

7. University of Macau

8. Macao

9. Department of Biology and Chemistry

10. City University of Hong Kong

11. Partner State Key Laboratory of Environmental and Biological Analysis

Abstract

An iridium(iii) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The limit of detection for TdT was 0.25 U ML⁻¹.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2015/CC/C5CC02790J

Reference43 articles.

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2. Improved detection of terminal transferase (TdT): The use of detergents on glutaraldehyde-fixed non-dehydrated cells prevents denaturation and diffusion artifacts

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4. Direct Fluorescence Detection of RNA on Microarrays by Surface-Initiated Enzymatic Polymerization

5. A TdT-mediated cascade signal amplification strategy based on dendritic DNA matrix for label-free multifunctional electrochemical biosensing

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