Assessment of shifted excitation Raman difference spectroscopy in highly fluorescent biological samples

Author:

Korinth Florian12,Shaik Tanveer Ahmed1ORCID,Popp Jürgen13,Krafft Christoph1ORCID

Affiliation:

1. Leibniz Institute of Photonic Technology and Member of Leibniz Research Alliance “Health Technologies”, 07745 Jena, Germany

2. Leibniz Institute for Astrophysics Potsdam and Member of Leibniz Research Alliance “Health Technologies”, 14482 Potsdam, Germany

3. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, 07743 Jena, Germany

Abstract

Common baseline corrections often fail for Raman spectra with high fluorescent background as observed in genipin crosslinked pericardium. An optimized SERDS approach suppresses the background effectively and gives high quality reconstructed spectra.

Funder

Leibniz-Gemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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