NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids-Reference-Cited by-同舟云学术

NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids

Published:2021 Issue:19 Volume:12 Page:6719-6725
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Mineev Konstantin S.¹²³⁴⁵^ORCID,Goncharuk Sergey A.¹²³⁴⁵,Goncharuk Marina V.¹²³⁴,Povarova Natalia V.¹²³⁴,Sokolov Anatolii I.¹²³⁴,Baleeva Nadezhda S.¹²³⁴,Smirnov Alexander Yu.¹²³⁴^ORCID,Myasnyanko Ivan N.¹²³⁴^ORCID,Ruchkin Dmitry A.¹²³⁴,Bukhdruker Sergey⁵⁶⁴⁷⁸,Remeeva Alina⁵⁶⁴,Mishin Alexey⁵⁶⁴^ORCID,Borshchevskiy Valentin⁵⁶⁴⁷⁸,Gordeliy Valentin⁵⁶⁴⁷⁸,Arseniev Alexander S.¹²³⁴,Gorbachev Dmitriy A.¹²³⁴,Gavrikov Alexey S.¹²³⁴,Mishin Alexander S.¹²³⁴^ORCID,Baranov Mikhail S.¹²³⁴⁹^ORCID

Affiliation:

1. Institute of Bioorganic Chemistry

2. Russian Academy of Sciences

3. 117997 Moscow

4. Russia

5. Moscow Institute of Physics and Technology

6. Dolgoprudny

7. Institute of Biological Information Processing (IBI-7: Structural Biochemistry)

8. Forschungszentrum Jülich GmbH

9. Pirogov Russian National Research Medical University

Abstract

We solved the structure of a fluorogen-activating protein FAST and synthesized the library of potential fluorogens. Using these data, we designed the shortest genetically encoded fluorescent tag among all known.

Funder

Russian Science Foundation

Russian Foundation for Basic Research

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D1SC01454D

Reference47 articles.

1. A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging

2. Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues