Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity-Reference-Cited by-同舟云学术

Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity

Published:2020 Issue:2 Volume:12 Page:149-161
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Silva Caio de Oliveira Gorgulho¹²³⁴,Teixeira Tallyta Santos¹²³⁴⁵,Rodrigues Kelly Barreto¹²³⁴,Souza Amanda Araújo¹²³⁴,Monclaro Antonielle Vieira¹²³⁴,Mendes Thaís Demarchi¹²³⁴,Ribeiro José Antônio de Aquino¹²³⁴,Gonçalves de Siqueira Félix¹²³⁴⁵,Fávaro Léia Cecília de Lima¹²³⁴,Abdelnur Patrícia Verardi¹²³⁴⁶^ORCID

Affiliation:

1. Brazilian Agricultural Research Corporation

2. Embrapa Agroenergy

3. Brasília

4. Brazil

5. Federal University of Tocantins

6. Federal University of Goiás

Abstract

Two new mass spectrometry methods, MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS, were developed for the characterization of cellulose-active lytic polysaccharide monooxygenases, expanding the analytical toolbox for the study of these enzymes.

Funder

Empresa Brasileira de Pesquisa Agropecuária

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AY/C9AY01774G

Reference27 articles.

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5. B. Westereng , J.Loose , G.Vaaje-kolstad , F. L.Aachmann , M.Sorlie and V. G. H.Eijsink , in Cellulases: Methods and Protocols. Methods in Molecular Biology , 2018 , pp. 219–246

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