Green method of synthesizing <scp>l</scp>-malate from <scp>d</scp>-glucose via CO2 fixation using an ATP-free in vitro synthetic enzymatic biosystem-Reference-Cited by-同舟云学术

Green method of synthesizing l-malate from d-glucose via CO₂ fixation using an ATP-free in vitro synthetic enzymatic biosystem

Published:2024 Issue:17 Volume:26 Page:9357-9370
ISSN:1463-9262
Container-title:Green Chemistry
language:en
Short-container-title:Green Chem.

Author:

Fan Lin¹²,Sun Shangshang¹,Zhang Zhidan³,Qin Yanmei¹,Jensen Peter Ruhdal⁴,You Chun¹²^ORCID

Affiliation:

1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin Airport Economic Area, Tianjin, 300308, China

2. University of Chinese Academy of Sciences Sino-Danish College, Beijing, China

3. Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308 Tianjin, China

4. Microbial Biotechnology & Biorefining National Food Institute, Kemitorvet, 201, 213, 2800 Kgs. Lyngby, Denmark

Abstract

An in vitro synthetic enzymatic biosystem (ivSEB) comprising five thermophilic enzymes with balanced NAD+/NADH was developed for the biomanufacturing of 2 moles of l-malate from 1 mole of d-glucose through the fixation of 2 moles of CO2.

Funder

National Basic Research Program of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/GC/D4GC01799D

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