Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release-Reference-Cited by-同舟云学术

Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

Published:2015 Issue:16 Volume:140 Page:5450-5453
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhou Lu¹²³⁴⁵,Shen Xiaoqiang¹²³⁴⁵,Sun Na¹²³⁴⁵,Wang Kewei¹²³⁴⁵,Zhang Yuanyuan¹²³⁴⁵,Pei Renjun¹²³⁴⁵

Affiliation:

1. Key Laboratory of Nano-Bio Interfacce

2. Division of Nanobiomedicine

3. Suzhou Insitute of Nano-Tech and Nano-Bionics

4. Chinese Academy of Science

5. Suzhou

Abstract

A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AN/C5AN01032B

Reference49 articles.

1. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.

2. Polynucleotide Kinase from Rat Liver Nuclear Extracts

3. Purification and properties of polynucleotide kinase of calf thymus

4. 17 Eukaryotic DNA Kinases

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