Label-free fluorescence light-up detection of T4 polynucleotide kinase activity using the split-to-intact G-quadruplex strategy by ligation-triggered and toehold-mediated strand displacement release

Author:

Zhou Lu12345,Shen Xiaoqiang12345,Sun Na12345,Wang Kewei12345,Zhang Yuanyuan12345,Pei Renjun12345

Affiliation:

1. Key Laboratory of Nano-Bio Interfacce

2. Division of Nanobiomedicine

3. Suzhou Insitute of Nano-Tech and Nano-Bionics

4. Chinese Academy of Science

5. Suzhou

Abstract

A label-free, fluorescence light-up detection method for T4 polynucleotide kinase activity has been developed using the split-to-intact G-quadruplex strategy.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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