A three-dimensional dynamic DNA walker-mediated branching hybridization chain reaction for the ultrasensitive fluorescence sensing of ampicillin

Author:

Jiang Long1,Qu Xiaonan2,Sun Wenyu3,Zhang Manru1,Wang Yeru3,Wang Yu3ORCID,Zhao Yijin3,Zhang Fenfen1,Leng Yuning1,Liu Su1ORCID,Yu Jinghua4,Huang Jiadong34ORCID

Affiliation:

1. School of Water Conservancy and Environment, University of Jinan, Jinan 250022, P.R. China

2. Domestic Waste Treatment Service Center of Lvye Ecological Park, Laixi City, Qingdao 266600, P.R. China

3. School of Biological Sciences and Technology, University of Jinan, Jinan 250022, P.R. China

4. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China

Abstract

The sensing system is composed of an Nt·Bbvcl-powered DNA walker blocked by the AMP aptamer, hairpin-shaped DNA track probes (TP) and four kinds of metastable hairpin probes as the substrates of bHCR.

Funder

Taishan Scholar Project of Shandong Province

Project of Shandong Province Higher Educational Science and Technology Program

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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