Estimation of biological variance in coherent Raman microscopy data of two cell lines using chemometrics

Author:

Junjuri Rajendhar12ORCID,Calvarese Matteo1ORCID,Vafaeinezhad MohammadSadegh123ORCID,Vernuccio Federico4ORCID,Ventura Marco45ORCID,Meyer-Zedler Tobias12ORCID,Gavazzoni Benedetta4,Polli Dario45ORCID,Vanna Renzo5ORCID,Bongarzone Italia6ORCID,Ghislanzoni Silvia6ORCID,Negro Matteo7ORCID,Popp Juergen123ORCID,Bocklitz Thomas12ORCID

Affiliation:

1. Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Strasse 9, 07745 Jena, Germany

2. Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743 Jena, Germany

3. Max Planck School of Photonics, Jena, Germany

4. Department of Physics – Politecnico di Milano, P.za L. da Vinci 32, 20133 Milano, Italy

5. Istituto di Fotonica e Nanotecnologie – CNR, P.za L. da Vinci 32, 20133 Milano, Italy

6. Department of Diagnostic Innovation, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Giacomo Venezian 1, 20133, Milano, Italy

7. Cambridge Raman Imaging Ltd, Cambridge, UK

Abstract

Broadband Coherent Anti-Stokes Raman Scattering (BCARS) is a valuable spectroscopic imaging tool forestimating the biological variance of cells in biomedical applications.

Funder

HORIZON EUROPE European Innovation Council

Horizon 2020 Framework Programme

Bundesministerium für Bildung und Forschung

Publisher

Royal Society of Chemistry (RSC)

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