Rapid and direct quantification of the SARS-CoV-2 virus with an ultrasensitive nanobody-based photonic nanosensor-Reference-Cited by-同舟云学术

Rapid and direct quantification of the SARS-CoV-2 virus with an ultrasensitive nanobody-based photonic nanosensor

Published:2022 Issue:5 Volume:1 Page:983-993
ISSN:2635-0998
Container-title:Sensors & Diagnostics
language:en
Short-container-title:Sens. Diagn.

Author:

Ruiz-Vega Gisela¹,Soler Maria¹^ORCID,Estevez M. Carmen¹,Ramirez-Priego Patricia¹^ORCID,Pazos Martalu D.¹,Noriega María A.²,Margolles Yago³,Francés-Gómez Clara⁴,Geller Ron⁴,Matusali Giulia⁵,Colavita Francesca⁵,di Caro Antonino⁵,Casasnovas José M.²^ORCID,Fernández Luis Angel³,Lechuga Laura M.¹^ORCID

Affiliation:

1. Nanobiosensors and Bioanalytical Applications group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST, and CIBER-BBN, Bellaterra, 08193, Barcelona, Spain

2. Department of Macromolecular Structure, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Darwin 3, Campus Cantoblanco UAM, 28049 Madrid, Spain

3. Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Darwin 3, Campus Cantoblanco UAM, 28049 Madrid, Spain

4. Institute for Integrative Systems Biology (I2SysBio), Universitat de Valencia-CSIC, 46980, Valencia, Spain

5. National Institute for Infectious Disease “L. Spallanzani” IRCCS, Via Portuense 292, 00149, Rome, Italy

Abstract

An innovative nanophotonic sensor using bioengineered nanobodies as receptors enables ultrasensitive COVID-19 detection and viral load determination in a 15 min assay without sample pretreatments.

Funder

Consejo Superior de Investigaciones Científicas

European Commission

Centres de Recerca de Catalunya

Agencia Estatal de Investigación

Ministero della Salute

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2022/SD/D2SD00082B

Reference42 articles.

1. The screening value of RT-LAMP and RT-PCR in the diagnosis of COVID-19: systematic review and meta-analysis