Dual-frequency impedance assays for intracellular components in microalgal cells

Author:

Tang Tao1ORCID,Liu Xun1,Yuan Yapeng2,Kiya Ryota1,Shen Yigang2ORCID,Zhang Tianlong13,Suzuki Kengo4,Tanaka Yo2ORCID,Li Ming3ORCID,Hosokawa Yoichiroh1,Yalikun Yaxiaer12

Affiliation:

1. Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan

2. Center for Biosystems Dynamics Research (BDR), RIKEN, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan

3. School of Engineering, Macquarie University, Sydney, 2109, Australia

4. Euglena Co. Ltd., Tokyo 108-0014, Japan

Abstract

The capabilities of impedance cytometry to detect intracellular components (i.e., volume and distribution) are investigated, and it is found that non-uniform intracellular distribution causes the tilt tendency of high-frequency impedance pulses.

Funder

Japan Society for the Promotion of Science

Australian Research Council

Amada Foundation

Nippon Sheet Glass Foundation for Materials Science and Engineering

Foundation for Nara Institute of Science and Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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