Programmable switching of CRISPR/Cas12a activity by adjusting guide RNA conformation for non-nucleic acid marker analysis and logic gate applications-Reference-Cited by-同舟云学术

Programmable switching of CRISPR/Cas12a activity by adjusting guide RNA conformation for non-nucleic acid marker analysis and logic gate applications

Published:2024 Issue:29 Volume:48 Page:13107-13118
ISSN:1144-0546
Container-title:New Journal of Chemistry
language:en
Short-container-title:New J. Chem.

Author:

Wang Huihui¹,Zhu Zhixue¹,Pan Huan¹,Guo Zhiqiang¹,Zhang Mingshuo¹,Meng Yanling¹,Yue Xudong¹,Wang Yu¹^ORCID,Liu Su²^ORCID,Yu Jinghua³^ORCID,Huang Jiadong¹³^ORCID

Affiliation:

1. School of Biological Sciences and Technology, University of Jinan, Jinan 250022, P. R. China

2. School of Water Conservancy and Environment, University of Jinan, Jinan 250022, P. R. China

3. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China

Abstract

A powerful and universal fluorescent sensing platform for the detection of non-nucleic acid analytes based on programmable switching of CRISPR/Cas12 activity via modulating crRNA conformation is presented.

Funder

Natural Science Foundation of Shandong Province

National Natural Science Foundation of China

Taishan Scholar Foundation of Shandong Province

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/NJ/D4NJ01502A

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