Minimizing the off-target frequency of the CRISPR/Cas9 system <i>via</i> zwitterionic polymer conjugation and peptide fusion-Reference-Cited by-同舟云学术

Minimizing the off-target frequency of the CRISPR/Cas9 system via zwitterionic polymer conjugation and peptide fusion

Published:2023 Issue:23 Volume:14 Page:6375-6382
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Han Yanjiao¹,Yuan Zhefan²^ORCID,Zhong Sijin Luo²,Xu Haoxian³,Jiang Shaoyi²^ORCID

Affiliation:

1. Molecular Engineering and Science Institute, University of Washington, WA 98195, USA

2. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA

3. Department of Material Science and Engineering, University of Washington, Seattle, WA, 98195, USA

Abstract

The off-target frequency of CRISPR/Cas9 system can be significantly decreased via zwitterionic polymer conjugation or (EK)n peptide fusion while maintaining a similar level of on-target gene editing activity.

Funder

University of Washington

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D2SC07067G

Reference37 articles.

1. CRISPR-Cas systems for editing, regulating and targeting genomes

2. Multiplex Genome Engineering Using CRISPR/Cas Systems

3. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

4. Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9

5. Expanding the Biologist’s Toolkit with CRISPR-Cas9

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