Protein sensing in living cells by molecular rotor-based fluorescence-switchable chemical probes-Reference-Cited by-同舟云学术

Protein sensing in living cells by molecular rotor-based fluorescence-switchable chemical probes

Published:2016 Issue:1 Volume:7 Page:301-307
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Yu Wan-Ting¹²³⁴,Wu Ting-Wei¹²³⁴,Huang Chi-Ling¹²³⁴,Chen I-Chia¹²³⁴⁵,Tan Kui-Thong¹²³⁴⁵

Affiliation:

1. Department of Chemistry

2. National Tsing Hua University

3. Hsinchu 30013

4. Republic of China

5. Frontier Research Center on Fundamental and Applied Sciences of Matters

Abstract

We introduce a general design to construct fluorescence-switching probes. Upon the interaction of the ligand with the protein, the crowded surroundings restrict the bond rotation of the fluorescent molecular rotor to trigger a strong fluorescence signal, which is reduced upon the addition of a competitive ligand or after protein degradation.

Funder

Ministry of Science and Technology

Ministry of Education

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/SC/C5SC02808F

Reference45 articles.

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