A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

Author:

Matela Gabriela1234,Gao Peng12345,Guigas Gernot1234,Eckert Antonia F.1234,Nienhaus Karin1234,Ulrich Nienhaus G.12345ORCID

Affiliation:

1. Institute of Applied Physics

2. Karlsruhe Institute of Technology (KIT)

3. Karlsruhe

4. Germany

5. Institute of Nanotechnology and Institute of Toxicology and Genetics

Abstract

We report an advanced monomeric far-red fluorescent marker protein, mGarnet2, which shows excellent performance as a live-cell fusion marker for microscopy as well as STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

Funder

Helmholtz Association

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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