A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems-Reference-Cited by-同舟云学术

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

Published:2020 Issue:40 Volume:10 Page:23931-23935
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Popescu Codruţa C.¹²³⁴⁵,Stoian Marius C.¹²³⁴⁵,Cucos Lia-Maria⁶⁷⁸^ORCID,Coman Anca G.¹²³⁴⁵,Radoi Antonio⁹¹⁰⁸,Paun Anca¹²³⁴⁵,Hădade Niculina D.¹¹¹²¹³⁸,Gautier Arnaud¹⁴¹⁵¹⁶¹⁷¹⁸^ORCID,Popescu Costin-Ioan⁶⁷⁸,Matache Mihaela¹²³⁴⁵^ORCID

Affiliation:

1. University of Bucharest

2. Faculty of Chemistry

3. Department of Organic Chemistry, Biochemistry and Catalysis

4. Research Centre of Applied Organic Chemistry

5. RO-050663 Bucharest

6. Institute of Biochemistry of the Romanian Academy

7. Bucharest

8. Romania

9. National Institute for Research and Development in Microtechnology – IMT Bucharest

10. Voluntari

11. Faculty of Chemistry and Chemical Engineering

12. Supramolecular Organic and Organometallic Chemistry Centre

13. ‘‘Babes--Bolyai’’ University

14. Université Clermont Auvergne

15. CNRS

16. Sigma Clermont

17. ICCF

18. F-63000 Clermont-Ferrand

Abstract

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC).

Funder

Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii

Universitatea din București

EEA Grants/Norway Grants

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/RA/D0RA02382E

Reference40 articles.

1. Guidelines to reach high-quality purified recombinant proteins

2. Exploiting the interactions between poly-histidine fusion tags and immobilized metal ions

3. Immobilized metal ion affinity chromatography

4. Immobilized Metal‐Ion Affinity Chromatography: Status and Trends

5. Potential for Using Histidine Tags in Purification of Proteins at Large Scale

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