Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2

Author:

Nawattanapaiboon Kawin12345ORCID,Pasomsub Ekawat67893,Prombun Photchanathorn1234,Wongbunmak Akanit1234,Jenjitwanich Akarawit1234,Mahasupachai Pantanat1234,Vetcho Purichaya1234,Chayrach Cholticha1234,Manatjaroenlap Natthapon1234,Samphaongern Chonchanok1234,Watthanachockchai Treewat67893,Leedorkmai Phonthanat67893,Manopwisedjaroen Suwimon1011934,Akkarawongsapat Radeekorn1011934,Thitithanyanont Arunee1011934ORCID,Phanchana Matthew1213934ORCID,Panbangred Watanalai1411934,Chauvatcharin Somchai1411934,Srikhirin Toemsak511934ORCID

Affiliation:

1. Zenostic Co.

2. Ltd

3. Bangkok 10400

4. Thailand

5. School of Materials Science and Innovation

6. Virology and Molecular Microbiology Unit

7. Department of Pathology

8. Faculty of Medicine Ramathibodi Hospital

9. Mahidol University

10. Department of Microbiology

11. Faculty of Science

12. Department of Molecular Tropical Medicine and Genetics

13. Faculty of Tropical Medicine

14. Department of Biotechnology

Abstract

RT-LAMP to detect SARS-CoV-2: in a positive sample, RT-LAMP leads to a color change from pink to yellow.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference23 articles.

1. World Health Organization , Coronavirus disease (COVID-19) Situation Report – 209 , 2020

2. World Health Organization , Novel Coronavirus – Thailand (ex-China), https://www.who.int/csr/don/14-january-2020-novel-coronavirus-thailand-ex-china/en/

3. Recent advances and perspectives of nucleic acid detection for coronavirus

4. A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses

5. Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR

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