Selection and colorimetric application of ssDNA aptamers against metamitron based on magnetic bead-SELEX

Author:

Xie Xicheng1,Huang Weiwen1,Shen Guoqing1234,Yu Hong12,Wang Lumei1234ORCID

Affiliation:

1. Shanghai Jiao Tong University, YunNan (Dali) Research Institute, Dali, Yunnan 671000, China

2. School of Agriculture and Biology, Key Laboratory of Urban Agriculture, Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China

3. Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, 800 Dongchuan Rd, Shanghai 200240, China

4. Shanghai Urban Forest Ecosystem Research Station, National Forestry and Grassland Administration, 800 Dongchuan Rd, Shanghai 200240, China

Abstract

A metamitron-specific ssDNA aptamer with high affinity was screened using magnetic bead-SELEX with the establishment of its new colorimetric assay.

Funder

National Key Research and Development Program of China

Major Science and Technology Projects in Yunnan Province

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference38 articles.

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2. W.Heri , F.Pfister , B.Carroll , T.Parshley and J. B.Nabors , Production, Development, and Registration of Triazine Herbicides , ed. H. M. LeBaron , J. E. McFarland and O. C. Burnside , 2008 , pp. 31–43

3. M.Doble , A.Kumar , M.Doble and A.Kumar , Biodegradation of Pesticides , 2005 , pp. 89–100

4. Assessment of the effects of the photosynthesis-inhibiting herbicides Diuron, DCMU, metamitron and metribuzin on growth and nitrogenase activity of Nostoc muscorum and a new cyanobacterial isolate, strain G4

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