Automated multiwell fluorescence lifetime imaging for Förster resonance energy transfer assays and high content analysis-Reference-Cited by-同舟云学术

Automated multiwell fluorescence lifetime imaging for Förster resonance energy transfer assays and high content analysis

Published:2015 Issue:10 Volume:7 Page:4071-4089
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Kelly Douglas J.¹²³⁴,Warren Sean C.¹²³⁴,Alibhai Dominic⁵⁶³⁴,Kumar Sunil¹²³⁴,Alexandrov Yuriy¹²³⁴,Munro Ian¹²³⁴,Margineanu Anca¹²³⁴,McCormack Jessica⁷⁸³⁹⁴,Welsh Natalie J.⁷⁸³⁹⁴,Serwa Remigiusz A.¹⁰⁶³⁴,Thinon Emmanuelle¹⁰⁶³⁴,Kongsema Mesayamas¹¹³¹²⁴,McGinty James¹²³⁴,Talbot Clifford¹²³⁴,Murray Edward J.¹³¹⁴⁴,Stuhmeier Frank¹⁵¹⁶¹⁷⁴,Neil Mark A. A.¹²³⁴,Tate Edward W.¹⁰⁶³⁴,Braga Vania M. M.⁷⁸³⁹⁴,Lam Eric W.-F.¹¹³¹²⁴,Dunsby Christopher¹²³⁴¹⁸,French Paul M. W.¹²³⁴

Affiliation:

1. Photonics Group

2. Department of Physics

3. Imperial College London

4. UK

5. Institute for Chemical Biology

6. Department of Chemistry

7. National Heart and Lung Institute

8. Faculty of Medicine

9. London SW7 2AZ

10. Chemical Biology Section

11. Department of Surgery and Cancer

12. London

13. Retroscreen Virology Ltd

14. London EC1 2AX

15. Pfizer Global Research and Development

16. Pfizer Limited

17. Sandwich, Kent

18. Centre for Histopathology

Abstract

An HCA-FLIM instrument is presented alongside exemplar oligomerisation, intermolecular and intramolecular FRET assays that require robust measurement of small lifetime changes.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AY/C5AY00244C

Reference64 articles.

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3. R. M. Clegg , in Laboratory Techniques in Biochemistry and Molecular Biology, ed. T. W. J. Gadella, Elsevier, 2009, vol. 33, pp. 1–57

4. Fluorescence Resonance Energy Transfer-Based Stoichiometry in Living Cells

5. Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell

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