Automated centrifugal microfluidic system for the preparation of adaptor-ligated sequencing libraries-Reference-Cited by-同舟云学术

Automated centrifugal microfluidic system for the preparation of adaptor-ligated sequencing libraries

Published:2024 Issue:2 Volume:24 Page:182-196
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Guo Jimin¹^ORCID,Brassard Daniel¹^ORCID,Adam Nadine²^ORCID,Verster Adrian J.³^ORCID,Shay Julie A.³,Miville-Godin Caroline¹,Janta-Polczynski Mojra¹,Ferreira Jason¹,Mounier Maxence¹,Pilar Ana V.²,Tapp Kyle²,Classen Adam²,Shiu Matthew¹,Charlebois Denis⁴,Petronella Nicholas³,Weedmark Kelly²^ORCID,Corneau Nathalie²,Veres Teodor¹

Affiliation:

1. Medical Devices Research Center, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC, J4B 6Y4, Canada

2. Bureau of Microbial Hazards, Microbiology Research Division, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0K9, Canada

3. Bureau of Food Surveillance and Science Integration, Bioinformatics High-Capacity Computing Laboratory, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0K9, Canada

4. Canadian Space Agency, 6767 Route de l'Aéroport, Saint-Hubert, QC J3Y 8Y9, Canada

Abstract

Using an advanced pneumatic centrifugal microfluidic technology and simple injected microfluidic devices, we demonstrate a complete ligation-based library preparation procedure and apply it to the characterization of foodborne pathogens.

Funder

Health Canada

Canadian Space Agency

National Research Council Canada

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2024/LC/D3LC00781B

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