Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids-Reference-Cited by-同舟云学术

Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids

Published:2022 Issue:26 Volume:58 Page:4247-4250
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Zhou Jing¹,Hu Jianyu²,Liu Rui³^ORCID,Wang Chaoqun¹,Lv Yi¹^ORCID

Affiliation:

1. Analytical & Testing Centre, Sichuan University, Chengdu 610064, China

2. Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta T6G 2G3, Canada

3. Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China

Abstract

A nucleic acid amplification-free method is proposed by integrating the self-amplification effect of CRISPR-Cas12a with the enhancement effect of the large number of detectable atoms inside each gold nanoparticle.

Funder

National Natural Science Foundation of China

Department of Science and Technology of Sichuan Province

Fundamental Research Fund of Shandong University

Sichuan University

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CC/D2CC00792D

Reference35 articles.

1. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction–Based SARS-CoV-2 Tests by Time Since Exposure

2. Rolling circle amplification for single cell analysis and in situ sequencing

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5. Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology

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