A microfluidic alternating-pull–push active digitization method for sample-loss-free digital PCR
Author:
Affiliation:
1. Department of Chemistry
2. University of Kansas
3. Lawrence
4. USA
5. Bioengineering Program
6. School of Engineering
7. University of Kansas Cancer Center
Abstract
A robust microfluidic alternating-pull–push active digitization method based on pneumatic valve actuation enables reproducible and highly efficient digitization of small-volume samples for dPCR.
Funder
National Institute of Biomedical Imaging and Bioengineering
National Cancer Institute
Publisher
Royal Society of Chemistry (RSC)
Subject
Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering
Link
http://pubs.rsc.org/en/content/articlepdf/2019/LC/C9LC00932A
Reference55 articles.
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2. Detection of KRAS Mutations in Circulating Tumor DNA by Digital PCR in Early Stages of Pancreatic Cancer
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4. Contribution of KRAS mutations and c.2369C > T (p.T790M) EGFR to acquired resistance to EGFR-TKIs in EGFR mutant NSCLC: a study on circulating tumor DNA
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