Comparison of the specificity and affinity of surface immobilised Affimer binders using the quartz crystal microbalance-Reference-Cited by-同舟云学术

Comparison of the specificity and affinity of surface immobilised Affimer binders using the quartz crystal microbalance

Published:2016 Issue:22 Volume:141 Page:6278-6286
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Weckman Nicole E.¹²³⁴,McRae Cassey⁵⁶⁴,Ko Ferrigno Paul⁵⁶⁴,Seshia Ashwin A.¹²³⁴⁷

Affiliation:

1. Nanoscience Centre

2. University of Cambridge

3. Cambridge CB3 0FF

4. UK

5. Avacta Life Sciences

6. Wetherby LS23 7FZ

7. Department of Engineering

Abstract

This study investigates the performance of surface bound Affimer proteins, comparing the affinity and specificity of different binders for closely related immunoglobulin molecules using the quartz crystal microbalance with dissipation monitoring (QCM-D).

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AN/C6AN01602B

Reference37 articles.

1. Measuring proteins on microarrays

2. Engineering novel binding proteins from nonimmunoglobulin domains

3. Antibodies in diagnostics – from immunoassays to protein chips

4. Recent advances in immobilization methods of antibodies on solid supports

5. Peptide Aptamer Microarrays: Bridging the bio–detector interface

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