Bio-SAXS of single-stranded DNA-binding proteins: radiation protection by the compatible solute ectoine-Reference-Cited by-同舟云学术

Bio-SAXS of single-stranded DNA-binding proteins: radiation protection by the compatible solute ectoine

Published:2023 Issue:7 Volume:25 Page:5372-5382
ISSN:1463-9076
Container-title:Physical Chemistry Chemical Physics
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Hallier Dorothea C.¹²³^ORCID,Smales Glen J.³^ORCID,Seitz Harald¹²^ORCID,Hahn Marc Benjamin³^ORCID

Affiliation:

1. Universität Potsdam, Institut für Biochemie und Biologie, 14476 Potsdam, Germany

2. Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), 14476 Potsdam, Germany

3. Bundesanstalt für Materialforschung und -prüfung (BAM), 12205 Berlin, Germany

Abstract

Small-angle X-ray scattering (SAXS) is used for structural determination of biomolecules in liquids. To prevent radiation damage during bio-SAXS at single-stranded DNA-binding proteins the OH-radical scavenger and compatible solute Ectoine is used.

Funder

Deutsche Forschungsgemeinschaft

Diamond Light Source

European Commission

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2023/CP/D2CP05053F

Reference52 articles.

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4. Limiting radiation damage for high-brilliance biological solution scattering: practical experience at the EMBL P12 beamline PETRAIII

5. A SAXS-based approach to rationally evaluate radical scavengers – toward eliminating radiation damage in solution and crystallographic studies

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2. Interaction of a Dimeric Single-Stranded DNA-Binding Protein (G5P) with DNA Hairpins. A Molecular Beacon Study;The Journal of Physical Chemistry B;2023-09-13

3. Accessing radiation damage to biomolecules on the nanoscale by particle-scattering simulations;Journal of Physics Communications;2023-04-01